effect of culture substrates on virulence of metarhizium anisopliae conidia and blastospores against sunn pest, eurygaster integriceps

virulence of blastospores and conidia of the fungus metarhizium anisopliae were investigatedon different instar nymphs and adults of sunn pest, eurygater integriceps. experiments werecarried out through a factorial arrangement in a completely randomized design of threereplications. the bioassays were performed through immersion method. results revealedsignificant differences among virulence of blastospores and of conidia produced in differentmedia.the highest vs the lowest total mortality percentages of the second instar nymphs wererecorded as 100 and 41.6% on rice bran vs wheat bran+ rice bran media respectively inblastopore treatments. as for conidia, the highest total mortality percentage of second instarnymphs was 100% on wheat bran, behnoush waste barley and rice flour media whilst thelowest total mortality percentage recorded as 86.1% on maize medium. minimum lt50 wasrecorded (2.3 days) on wheat bran + yeast extract medium in the second instar nymphs whilstthe highest lt50 recorded (10.1 days) on wheat bran+ rice bran extract medium for the fourthinstar nymphs in blastopore treatments. minimum lt50 was recorded (4.7 days) on the ricebran medium and at second instar nymphs while the highest recorded (13.1 days) for the fourthinstar nymphs by conidia harvested from non-broken rice medium.